Macromolecular crowding experiments.

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bigglesworth
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Macromolecular crowding experiments.

Postby bigglesworth » Wed May 11, 2011 10:02 am UTC

I'm doing my undergrad lab project on the CD machine, essentially, and I was thinking of using macromolecular crowding agents to see the effect of those on the folding and unfolding of the protein I'm using. Other people (notably John Ellis) have done this before, but not with the protein I'm using (which is why I'm considering doing it in my project).

Does anyone here have any experience with using macromolecular crowding agents with proteins? Any advice on what works and what doesn't?
Generation Y. I don't remember the First Gulf War, but do remember floppy disks.

opsomath
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Re: Macromolecular crowding experiments.

Postby opsomath » Thu May 12, 2011 6:57 pm UTC

Cool. What will you use, PEG?

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bigglesworth
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Re: Macromolecular crowding experiments.

Postby bigglesworth » Thu May 12, 2011 7:02 pm UTC

Probably going for Ficoll 70, since someone in the lab has used that before.
Generation Y. I don't remember the First Gulf War, but do remember floppy disks.


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